Viola odorata known as sweet violet, is considered as rare and endemic perennial plant to Europe and Asia and belongs to the genus viola, which includes more than 400 species. Sweet violet is one of the most important medicinal plant used widely in traditional therapy for various ailments like bronchitis, common digestive disorders, post-operative tumor metastasis, diabetes and cancer. Phytochemically, different groups of compounds have been isolated from various species of this genus like cyclotides, flavonoids, alkaloids and triterpenoids. Some of them already have been scientifically accepted as antifungal, antibacterial, anticancer, antioxidant, antiasthmatic, anti-inflammatory, anti-HIV and antipyretic agents. Recently, a new herbal drug “ODORATA” which formulated into syrup was synthesized based on active constituents of V. odorata. The germination rate of this medicinal plant is low because of sever seed dormancy. So, we searched for an efficient direct shoot regeneration system for clonal propagation of V. odorata that can be used for large-scale production of true-to-type plants and for improving the species using genetic engineering techniques.