The family Valerianaceae includes 400 species of which about 200 belong to Valeriana genus. Valeriana officinalis, commonly known as valerian, is native to Europe and Asia and has economic importance as a medicinal plant. Roots or rhizomes are the main parts of the plant that are medicinally used. Now, valerian extracts are considered as dietary supplements, which are formulated into tablets or soft gelatin capsules, commonly used as anti-HIV, sleep aid, antidepressant and antihypertensive agents. Valerian is traditionally propagated through seeds or root divisions. Fresh seeds are only 30% viable, and this number goes down after the first year of storage. Root division is also suffering from root rot and white mold infections. Besides, chemical constituents in valerian plants vary depending on different sources and growing conditions, processing methods and storage conditions. Developing a method for mass propagation of this valuable medicinal plant is essential to improve the quality and quantity of raw plant material to guarantee its commercial production. So, an effective in vitro method was developed for plant propagation of Valeriana officinalis by node culture technique